A spectrum of mutations induced by crotonaldehyde in shuttle vector plasmids propagated in human cells
نویسندگان
چکیده
منابع مشابه
A spectrum of mutations induced by crotonaldehyde in shuttle vector plasmids propagated in human cells.
A spectrum of crotonaldehyde-induced mutations in the supF gene of the shuttle vector plasmid pMY189 replicated in human fibroblast cells was examined. Base sequence analysis of 104 plasmids with mutations in the supF gene revealed that the majority of the mutations were base substitutions (85%) and the rest were frameshifts (15%). A single base substitution was most frequently found (47%), whi...
متن کاملMutagenic events induced by 4-hydroxyequilin in supF shuttle vector plasmid propagated in human cells.
Increased incidence of breast, ovarian and endometrial cancers are observed in women receiving estrogen replacement therapy (ERT). Equilin and equilenin are the major components of the widely prescribed drug used for ERT. These equine estrogens are metabolized primarily to 4-hydroxyequilin (4-OHEQ) and 4-hydroxyequilenin, respectively, which are autoxidized to react with DNA, resulting in the v...
متن کاملRestricted ultraviolet mutational spectrum in a shuttle vector propagated in xeroderma pigmentosum cells.
A shuttle vector plasmid, pZ189, carrying a bacterial suppressor tRNA marker gene, was treated with ultraviolet radiation and propagated in cultured skin cells from a patient with the skin-cancer-prone, DNA repair-deficient disease xeroderma pigmentosum and in repair-proficient cells. After replication in the human cells, progeny plasmids were purified. Plasmid survival and mutations inactivati...
متن کاملMultiple point mutations in a shuttle vector propagated in human cells: evidence for an error-prone DNA polymerase activity.
Mutagenesis was studied at the DNA-sequence level in human fibroblast and lymphoid cells by use of a shuttle vector plasmid, pZ189, containing a suppressor tRNA marker gene. In a series of experiments, 62 plasmids were recovered that had two to six base substitutions in the 160-base-pair marker gene. Approximately 20-30% of the mutant plasmids that were recovered after passing ultraviolet-treat...
متن کاملAnalysis of W-Methyl-Af-nitrosourea-induced Mutations in a Shuttle Vector Plasmid Propagated in Mouse O6-Methylguanine-DNA Methyltransferase- deficient Cells in Comparison with Proficient Cells1
A shuttle vector plasmiti. pYZ289, was constructed from the pZ189 plasmid and polyoma virus DNA. The plasmid contains a supF gene as a marker of mutation and can replicate in both Escherìchiacoli and mouse cells. The pYZ289 plasmids treated with /V-methyl-A'-nitrosourea were passed through mouse cells originating from skin tumors, which are either proficient (III IS) or deficient (III 8) in O'...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Carcinogenesis
سال: 1998
ISSN: 1460-2180
DOI: 10.1093/carcin/19.1.69